Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit Green Features, 100 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
100 Reactions
Order number :
4402956
Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit Green Features, 100 Reactions
Ambion® Fast SYBR® Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. The kit includes sufficient reagents for 400 reactions. The Fast SYBR® Green Cells-to-CT™ Kit contains AmpliTaq® Fast DNA Polymerase, UP, which allows instant hot start, thus minimizing non-specific product formation with fast real-time PCR.
Features
- Includes optimized reagents to work efficiently, right out of the box
- Simple, effective Cells-to-CT™ technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
- Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA
- For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
- Detection Method: SYBR
- Green Features: Fewer resources used and less waste, Less hazardous
- Hot Start: Built-In Hot Start
- Label or Dye: SYBR™ Green
- Passive Reference Dye: ROX (Pre-mixed)
- Polymerase: AmpliTaq™ Fast DNA Polymerase
- Primer Probe Compatibility: Unlabeled Oligos
- Purity: UP (Ultra Pure)
- Reverse Transcriptase: M-MLV
- Shipping Condition: Dry Ice
- Sample Type: Cells
Contents
- Stop Solution: 500 μL
- DNase I: 55 μL
- 20X RT Enzyme Mix: 275 μL
- Lysis Solution: 5.5 mL
- 2X SYBR® RT Buffer: 5.5 mL
- Fast SYBR® Green PCR Master Mix: 5 mL
- Nuclease-free Water: 2 x 5 mL
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