Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit
On demand
Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit
The
resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR
(qPCR)-based system for the detection of host-cell DNA from HEK293 cell
lines used in the development of gene therapy, cell-based vaccine, and
similar biotherapeutics. Reliable and rapid, the resDNASEQ system
enables sensitive and specific quantitation of HEK293 cell DNA*. This
performance helps ensure a high degree of confidence in quantitation
data obtained from a broad range of sample types—from in-process samples
with different sample matrices to purified final product—whether the
sample contains high molecular weight or sheared DNA.
This
kit is bundled together with sample preparation reagents in the form of
a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for
highly efficient residual DNA recovery from complex mixtures of
proteins, buffers, and salts
Features
- Highly sensitive quantitation using proven TaqMan real-time qPCR technology
- Consistent performance across the expected range of DNA fragment sizes
- Includes master mix, TaqMan primer/probe mix, and HEK293 genomic DNA standard
Contents
resDNASEQ HEK293 DNA Control
- HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
- DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use
resDNASEQ HEK293 DNA Real-Time PCR Reagents
- 2X Environmental Master Mix, 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
- 10X HEK293 DNA Assay Mix, 300 μL, store at –15 to –25°C, protect from light
- Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
PrepSEQ Nucleic Acid Extraction Kit
- Lysis Buffer (2 bottles, 50 mL/bottle)
- Binding Solution/Isopropanol (1 empty bottle)
- Wash Buffer Concentrate (2 bottles, 26 mL/bottle)
- Elution Buffer (1 bottle, 25 mL)
- Proteinase K Buffer( 1 bottle, 50 mL)
- Magnetic Particles (2 tubes, 1.5 mL/tube)
- Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below -20°C
- PK Buffer II (1 bottle, 11 mL)
- Store all at room temperature unless otherwise indicated.
PrepSEQ Residual DNA Sample Preparation Kit
- Proteinase K (20 mg/mL) (1 tube, 1.25 mL)
- Yeast tRNA (10 mg/mL) (1 tube, 0.5 mL)
- Glycogen (5 mg/mL) (2 tubes, 1.0 mL/tube)
- Store all at or below -20°C.
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