Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase, 200
On demandThermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase, 200
Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. It provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription. For the first strand cDNA synthesis the kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT. The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C). The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.
Features
- Integrated gDNA removal step
- High yields of full length cDNA up to 20 kb
- Efficient cDNA synthesis at wide temperature range from 42°C to 65°C
- Increased synthesis rate—complete cDNA synthesis in 15-30 minutes
- High sensitivity and specificity
Contents
- Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
- 5X Reaction Mix (reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers)
- dsDNase
- 10X dsDNase Buffer
- Nuclease-free water
There are no report
You May Also Like
Applied Biosystems™ ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well
$ 62
In stock
Applied Biosystems™ QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL
$ 1,430 $ 1,573
In stock
Thermo Scientific™ Luminaris Probe qPCR Master Mix, 1250 Reactions
$ On demand
On demand
Thermo Scientific™ ABsolute Blue QPCR Mix, with separate ROX vial, 400 Reactions
$ On demand
On demand
Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, high ROX, 250 Reactions
$ On demand