Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kit

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kit

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform used in the development of gene therapy, cell-based vaccines, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of Sf9 host cell DNA and baculovirus DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples with different sample matrices to purified final product—whether the sample contains high-molecular-weight or sheared DNA.

Features

• Duplex assay design to quantitate both Sf9 and baculovirus residual DNA 
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology 
• Consistent performance across the expected range of DNA fragment sizes 
• Includes master mix, TaqMan primer/probe mix, and Sf9 and baculovirus DNA standard

Contents

resDNASEQ Sf9 and Baculovirus DNA Control

• Multiplex DNA Control (Sf9 DNA Control, 30 ng/μL; Baculovirus DNA Control, 30 ng/μL), 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2–8°C after first useres

DNASEQ Real-Time PCR Reagents

• 2X Environmental Master Mix, 2 x 0.75 mL, store at –15 to –25°C before first use and 2–8°C after first use, protect from light
• 10X Sf9 + Baculovirus DNA Assay Mix , 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2–8°C after first use