Applied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kit
On demandApplied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kit
The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.
Features
- Highly sensitive quantitation using proven TaqMan real-time qPCR technology
- Offers consistent performance across the expected range of DNA fragment sizes
- Integrated system includes sample preparation kit, master mix, TaqMan primer/probe mix, and Vero genomic DNA standard
- Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices
Contents
resDNASEQ Vero DNA Real-Time PCR Reagents:
- 2X Environmental Master Mix (2 tubes, 0.75 mL per tube). Store at -15 to -25°C before first use, protect from light.
- 10X Vero DNA Assay Mix (1 tube, 300 µL). Store at -15 to -25°C, protect from light.
- Negative control water (1 tube, 1.0 mL). Store at -15 to -25°C before first use and 2–8°C after first use.
resDNASEQ Vero DNA Control:
- Vero DNA Control (1 tube, 40 µL, 30 ng/µL). Store at -15 to -25°C.
- DNA Dilution Buffer (1 bottle, 7 mL). Store at -15 to -25°C before first use and 2–8°C after first use
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