Applied Biosystems™ Shrimp Alkaline Phosphatase (SAP), 5000 Unites

Applied Biosystems™ Shrimp Alkaline Phosphatase (SAP), 5000 Unites

Shrimp Alkaline Phosphatase (SAP) is a high specific activity, heat-labile alkaline phosphatase  purified from a recombinant source and originally isolated from Pandalus borealis (arctic shrimp).  SAP is useful in many molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents relegation of linearized plasmid DNA. SAP may also be used to treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.

Shrimp Alkaline Phosphatase has approximately the same specific activity as Calf Intestinal Alkaline Phosphatase (CIAP), and like CIAP, is active in virtually all restriction enzyme reaction buffers. Unlike CIAP, Shrimp Alkaline Phosphatase is completely and irreversibly inactivated by heating reactions at 65°C for 15 min.

Shrimp Alkaline Phosphatase is particularly useful in preparing PCR products for applications involving sequencing, SNP analysis or labeling methods. Typically, excess dNTPs remaining after PCR interfere with subsequent enzymatic reactions involving DNA synthesis. SAP dephosphorylates all of the remaining dNTPs from the PCR mixture in one easy step.

Storage Buffer: 25mM Tris-HCl (pH 7.5), 1mM MgCl2, 50% glycerol

Features

• 100% heat-inactivated in 15 min at 65°C
• Significantly improved storage stability at lower temperatures 
• Very high specific activity 
• Removes 5'-phosphates from DNA, RNA, dNTPs, and proteins 
• Purified from a recombinant source
• May be added directly to restriction enzyme digests 
• No vector purification necessary 
• Requires no supplemental zinc or other additives for activity 
• Works direct in many different buffers 
• Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis