Applied Biosystems™ TaqMan™ Antibiotic Resistance Assay

Applied Biosystems™ TaqMan™ Antibiotic Resistance Assay

Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial species and antibiotic resistance genes. These assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features

• Easy to use—just add DNA and master mix and run the qPCR—no melt curves required 
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low-abundance microbial targets
• Accurate—identify microbial targets with high accuracy of quantitation
• Extensive content—over 300 pre-designed microbe detection assays and 100 pre-designed antibiotic resistance assays currently available
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for closely related microbial species and subtypes
• Fast delivery—assays shipped in as little as 1–2 days 

Contents

• 1 tube containing 20X mix of pre-formulated assay (1 probe and 2 primers).