Applied Biosystems™ TaqMan™ Protein Assays Open Kit

Applied Biosystems™ TaqMan™ Protein Assays Open Kit

The TaqMan™ Protein Assays Open Kit contains the TaqMan™ Protein Assays Oligo Probe Kit (4448549) and the TaqMan™ Protein Assays Buffer Kit (4448571) required for making TaqMan™ Protein Assay probes, along with the customer-supplied biotinylated antibodies. You supply the biotinylated antibodies and add them to streptavidin-oligonucleotides (prox-oligos) to make the 2 antibody-oligonucleotide assay probes utilized in binding. The resulting assay probes are utilized to help quantitate protein expression using gold standard TaqMan™ 5' nuclease chemistry with a workflow and sample quantity similar to our TaqMan™ Gene Expression and MicroRNA Assays. As the protein expression results are obtained on the same analytical platform, these revolutionary assays enable direct correlation of mRNA and⁄or miRNA expression to protein expression.

For use with equipment: 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode

Features

• Small Sample Size – use up to 500 times less sample input compared to traditional methods
• Sensitive Detection – directly measure in vitro protein-protein interactions with only a few thousand cells
• Revolutionary – directly correlate mRNA and⁄or microRNA to protein
• Simple Workflow – 1-step lysis sample preparation, universal protocol
• Quantitative – protein marker expression fold changes detected

Contents

• The TaqMan™ Protein Assays Open Kit contains the TaqMan™ Protein Assays Oligo Probe Kit (4448549) and the TaqMan™ Protein Assays Buffer Kit (4448571)for making TaqMan™ Protein Assay probes.