Applied Biosystems™ ExoSAP-IT Express PCR Product Cleanup with Tracking Dye, 1920 Reaction

Applied Biosystems™ ExoSAP-IT Express PCR Product Cleanup with Tracking Dye, 1920 Reaction  

It is necessary to remove excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing. An enzymatic cleanup method offers precision and speed over technologies such as spin columns or beads. ExoSAP-IT Express reagent consists of two hydrolytic enzymes, a modified Exonuclease I and Shrimp Alkaline Phosphatase (SAP), in a specially formulated buffer. It is used to remove excess primers and dNTPs from a PCR mixture with no interference in downstream applications. This unique, highly stable, one tube solution allows for greater confidence and superior sequencing results.

Features

• 5 minute protocol — Fastest turnaround time to results
• One-tube, one-step PCR cleanup — Add ExoSAP-IT Express directly to PCR product
• Novel enzyme technology — Irreversibly inactivated in just one minute at 80°C
• Conserve PCR samples — 100% recovery of PCR products, regardless of amplicon length
• Eliminate spin columns or beads — Significantly decreases time and errors while increasing yield
• Compatible — No interference in downstream applications
• Scalable — Treat a wide range of volumes
• Multiple formats — Single tube, 8 tube strip, and 96-well plate formats offer flexibility from manual pipetting to automated workflows
• Go green — Generate less waste with a single-tube solution to PCR cleanup
• Optional tracking dye — Never lose track of reagent addition during pipetting
  
  

Contents

• Single Tube
• 8 Tube strip
• 96-well Plate