Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of human cell DNA*. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.
The resDNASEQ Human Residual DNA Quantitation Kit provides highly sensitive detection of human DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of human DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Features

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and human genomic DNA standard
  
  

Contents

• 2X Environmental Master Mix: 2 tubes, 0.75 mL per tube
• 10X Human DNA Assay Mix: 1 tube, 300 µL
• Negative control water: 1 tube, 1.0 mL
• Human DNA Control: 1 tube, 40 µL
• DNA Dilution Buffer: 1 bottle, 7 mL
• Lysis Buffer: 2 bottles, 50 mL/bottle
• Binding Solution/Isopropanol: 1 empty bottle
• Wash Buffer Concentrate: 2 bottles, 26 mL/bottle
• Elution Buffer: 1 bottle, 25 mL
• Proteinase K Buffer: 1 bottle, 50 mL
• Magnetic Particles: 2 tubes, 1.5 mL/tube
• Proteinase K (20 mg/mL): 1 tube, 1.25 mL
• PK Buffer II: 1 bottle, 50 mL
• Proteinase K (20 mg/mL): 1 tube, 1.25 mL
• Yeast tRNA (10 mg/mL): 1 tube, 0.5 mL
• Glycogen (5 mg/mL): 2 tubes, 1.0 mL/tube

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone without the sample preparation kit, please see resDNASEQ Human Residual DNA Quantitation Kit.