Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kit
On demandApplied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kit
This quantitative PCR (qPCR)-based kit—part of the resDNASEQ® Quantitative CHO DNA System—enables you to detect sub-picogram amounts of residual DNA from the Chinese hamster ovary (CHO) cell line in under 5 hours. The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ® sample preparation and TaqMan®-based quantitation of residual DNA.
The resDNASEQ® Quantitative CHO DNA System provides highly sensitive detection of CHO DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan® technology allows you to test samples with variable levels of CHO DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts. Figure 1 demonstrates the range and sensitivity of the assay. Linearity is demonstrated by analysis of CHO cell standard DNA ranging from 0.3 ng to 3 fg.
Features
- Easy to use, results in under 5 hours
- Specific to CHO DNA, no cross-reactivity with unrelated DNA
- Part of an integrated system that includes sample preparation, TaqMan® assay and master mix, standard DNA, instrument, and software
- Protocol available for automated sample preparation
- Flexible sample throughput allows you to perform rigorous DNA clearance studies
- Host Cell: CHO
- PCR Method: qPCR
- Primer Probe Compatibility: TaqMan™
- Product Line: resDNASEQ™
- Product Type: Quantitative CHO DNA Kit
- Reaction Speed: Standard
- Shipping Condition: Dry Ice
- Detection Method: Primer-Probe Detection
- Format: Kit
- Label or Dye: FAM
- Sample Type: DNA (Genomic)
Contents
- CHO DNA Control 30 ng/μL: 40 μL
- DNA Dilution Buffer (DDB): 7 mL
- 2X Environmental Master Mix: 2 × 0.75 mL
- 10X CHO DNA Real-Time PCR Assay Mix: 300 μL
- Negative Control (water): 1.0 mL
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