Invitrogen™ MessageAmp™ II aRNA Amplification Kit, 100 Reactions

Invitrogen™ MessageAmp™ II aRNA Amplification Kit, 100 Reactions

The MessageAmp II aRNA Amplification Kit amplifies as little as 100 pg of input RNA for GeneChip analysis. 

Expression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide/scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This hurdle is usually overcome by using T7 RNA polymerase-driven transcription of cDNA (obtained from total RNA samples) to amplify sufficient amounts of cRNA. This amplification method is the basis of the MessageAmp II aRNA Amplification Kit.

Features

• Increased amplification—up to 5,000-fold
• Greater sensitivity due to increased yields of full-length cDNA
• GeneChip analysis with as little as 100 ng input RNA
• Efficient labeling produces high percent present calls and low 3'/5' ratios
• Includes ArrayScript RT, a specifically engineered M-MLV reverse transcriptase
• Optimized second strand cDNA synthesis reaction

Contents

• The MessageAmp™ II aRNA Amplification Kit contains T7 Oligo(dT) Primer, ArrayScript™ RT, RNase Inhibitor,10X First-Strand Buffer, dNTP Mix, Second-Strand Buffer, DNA Polymerase, RNase H, T7 Enzyme Mix, T7 10X Reaction Buffer, T7 ATP (75 mM), T7 CTP (75 mM), T7 GTP (75 mM), T7 UTP (75 mM), Second Round Primers, and Control RNA (1 mg⁄mL HeLa total RNA) which are all stored at –20°C. The Wash Buffer, cDNA Binding Buffer, aRNA Binding Buffer, aRNA Filter Cartridges, aRNA Collection Tubes, cDNA Filter Cartridges, cDNA Elution Tubes, Nuclease-free Water, and detailed Instruction Manual are stored at room temperature.