Axygen® AxyPrep MicroRNA (miRNA) Miniprep Kit, 250 preps

Axygen® AxyPrep MicroRNA (miRNA) Miniprep Kit, 250 preps

The AxyPrep miRNA Miniprep Kit represents a new approach for purification of Micro (miRNA) RNA directly from animal or plant tissue and cultured cells. It produces a purified cellular RNA subfraction which is highly enriched for lower molecular weight species, such as miRNA, and siRNAs. This Kit is designed to eliminate the problems associated with other spin column type RNA Kits, such as column clogging and incomplete purification. Proteins and genomic DNA are precipitated and removed from the cell lysate. Then, larger RNAs, such as mRNA, 18 s and 28 s rRNA and residual DNA are removed by binding to a spin/vacuum column. miRNA and siRNAs remain unbound and are recovered by the addition of isopropanol and centrifugation. Axygen® AxyPrep MicroRNA (miRNA) Miniprep Kit, 250 Preps per Kit

Features

• Rapid spin and vacuum protocols
• For animal tissues, plants tissues, cultured cells
• No column clogging
• No organic extraction or proteinase digestion
• Enriches for miRNAs andsiRNAs

Contents

• Spin/vac mini column: 250
• 2 ml microfuge tube: 250
• 1.5 ml microfuge tube: 250
• Buffer R-I: 125 ml
• Buffer G-B: 120 ml
• Buffer R-II: 50 ml
• Buffer TE(DNase&RNase-free): 120 ml