Axygen® AxyPrep Plasmid Kit, 4 x 96 preps

Axygen® AxyPrep Plasmid Kit, 4 x 96 preps

The AxyPrep-96 Plasmid Kit represents a true high-throughput system for the purification of plasmid or cosmid DNA in a 96-well format. This product is designed to produce up to 20 µg of highly purified plasmid or cosmid DNA (per well) from up to 1.3 mL of bacterial cultures in 96-well format. Two plates rapidly and efficiently process the bacterial lysate, using either vacuum or centrifugation. The Lysate Filtration Plate efficiently removes bacterial cellular debris and transfers the clarified lysate directly into the Plasmid Plate for desalting. The plasmid is then eluted into a small volume of buffer or deionized water. The highly-purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and is suitable for any application, including: automated fluorescent sequencing on the ABI PRISM DNA Analyzers, in vitro transcription, restriction analysis and PCR. A vacuum manifold and vacuum source are required.Axygen® AxyPrep-96 Plasmid Kit, 4 x 96 prep per Kit

Features

• High-throughput, 96-well plate format
• Two-plate system ( lysate filtration and plasmid binding
• Up to 20 µg of highly purified bacterial plasmid per well
• Rapid vacuum and centrifuge protocols
• No alcohol precipitation
• Suitable for automated fluorescent sequencing
• Automation-compatible plasticware

Contents

• 96-well 2.2 ml growblock: 4
• 96-well 1.6 ml growblock: 2
• 96-well Filtration plate: 4
• 96-well DNA plate: 4
• 96-well V-bottom sample plate: 6
• BF-400 breathable film: 4
• Adhesive film: 10
• Silicone mat: 8
• RNase A: 300 Pl
• Buffer S1: 135 ml
• Buffer S2: 135 ml
• Buffer S3: 200 ml
• Buffer W1: 230 ml
• Buffer W2 concentrate: 4×72 ml
• Eluent: 50 ml