Invitrogen™PureLink™ 96 HQ Plasmid DNA Purification Kit

Invitrogen™PureLink™ 96 HQ Plasmid DNA Purification Kit

The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can beused with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.

Features

• Ability to isolate up to 10 µg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handlingworkstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection
• For Use With (Equipment): Automated Liquid Handling Workstation
• Purification Target: Plasmid DNA
• Sample Type: Bacterial Culture
• High-throughput Compatibility: High-throughput Compatible
• Prep Scale: < 100 µg (Small-Scale) Plasmid DNA

Contents

• Resuspension Buffer (R1): 120 mL
• Lysis Buffer (L1): 120 mL
• Neutralization/Binding Buffer (B1): 2 × 85 mL
• Wash Buffer (W1): 120 mL
• Elution Buffer; 10 mM Tris-HCl, pH 8.5 (E1): 70 mL
• RNase A: 12 mg
• PureLink™ HQ 96 Clarification Plate: 4
• PureLink™ HQ 96 Binding Plate: 4
• Receiver Plate: 4
• Adhesive Foil for plates: 20