CleanPlex® TMB 500 Panel For Illumina®, 6rxn
On demandCleanPlex® TMB 500 Panel For Illumina®, 6rxn
The CleanPlex® TMB 500 Panel is a targeted resequencing assay designed to enable rapid and accurate assessment of tumor mutational burden and mutation profile. The panel is expertly curated using the latest research findings to target 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets. The panel covers 1.64 Mb of genomic content (1.19 Mb exonic) and targets both single nucleotide variants (SNVs) and insertion-deletion mutations (indels). Two pools of multiplex PCR primers are utilized to construct target-enriched libraries from either formalin-fixed paraffin-embedded (FFPE) DNA from tumor samples or high-quality gDNA from blood samples. Starting with just 20 ng of DNA (10 ng per primer pool), sequencing-ready libraries can be prepared using a streamlined workflow in just 6 hours, allowing samples to be sequenced on the same day.
Contents
The CleanPlex TMB 500 Panel contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A 96rxn, Set B 96rxn for sample pooling, CleanMag®Magnetic Beads and CleanMag® Magnetic Rack or CleanMag® Magnetic Plate for bead purification and size selection are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.
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