Ion Torrent™ Oncomine™ IGH FR1-J Assay, DNA

Ion Torrent™ Oncomine™ IGH FR1-J Assay, DNA

The Oncomine IGH FR1-J Assay is a targeted next-generation sequencing (NGS) assay for detection of somatic hypermutation (SHM) in the variable region of the immunoglobulin heavy chain of B-cell receptors. SHM increases the affinity of the B-cell receptor for antigen, post-VDJ recombination, and is frequently a region of interest in CLL research. Primers have been specifically designed to target framework 1 and joining gene regions of the immunoglobulin heavy chain, resulting in an amplicon ∼300 bp in length. When combined with Ion Reporter Software, you can easily analyze your data and create reports within a short period of time.

For use with equipment: GeneStudio.

Features

• Sequencing of FR1-J region of IGH chain
• Detects SHM in CLL samples with high degree of sensitivity
• Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots

Contents

• Oncomine IGH FR1-J Panel
• dNTP mix
• Ion AmpliSeq Library Plus Kit