Corning® 10 mL Matrigel® Matrix for Organoid Culture, Phenol Red-free, LDEV-free

Corning® 10 mL Matrigel® Matrix for Organoid Culture, Phenol Red-free, LDEV-free

Corning Matrigel Matrix for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture. Verified to support both mouse and human organoid growth and differentiation from healthy and/or diseased tissue, each lot has a specific elastic modulus value indicating the stiffness of the gel formed and is qualified to form stable 3D domes commonly used in organoid culture protocols and is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality:

• Tested for ability to form and maintain stable 3D domes/droplets on multi-well plates.
• Elastic Modulus (matrix stiffness) measured using a rheometer.
• Protein concentrations determined by Lowry method
• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

Preparation and Storage, Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.