GeneJET RNA Purification Kit, 50 Preps

GeneJET RNA Purification Kit, 50 Preps

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Features

• Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria.
• Efficient – high yields of total RNA.
•  Fast – 15 minutes after lysis step.
• Pure – an A260/280 ratio > 1.9
• Convenient – spin columns are capped and assembled with collection tubes.
• Concentration: 10X

Contents

• Proteinase K: 600 μL
• Lysis Buffer: 40 mL
• Wash Buffer 1 (concentrated): 40 mL
• Wash Buffer 2 (concentrated): 23 mL
• Water, nuclease-free: 30 mL
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes (2 mL): 50
• Collection Tubes (1.5 mL): 50
• Collection Tubes (2 mL): 50