Gibco™ Bac-to-Bac™ HBM TOPO™ Secreted Expression System
On demand
Order number :
A11339
Gibco™ Bac-to-Bac™ HBM TOPO™ Secreted Expression System
Bac-to-Bac baculovirus expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells.
Features
- Expression of secreted proteins—The Bac-to-Bac HBM TOPO vector contains the honeybee melittin (HBM) secretion signal to enable secreted protein expression
- Flexibility—The Bac-to-Bac HBM TOPO vector contains a C-terminal His-tag with a TEV cleavage site to enable easy purification of His-tagged proteins with nickel-chelating resins (such as the Invitrogen ProBond Purification System) and generation of native proteins with the aid of the Invitrogen AcTEV Protease
- Fast cloning, easy screening—Blunt TOPO cloning technology enables cloning of blunt-end PCR products into the pFastBac TOPO vector in five minutes
- High transfection efficiency with ExpiFectamine Sf Transfection Reagent
- Time-saving expression bacmid
- Easy colony screening
- High protein expression—The pFastBac vector uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell line such as Sf9, Sf21, and High Five cells
Contents
- Vector kit for 20 TOPO cloning reactions
–pFastBac/HBM-TOPO vector containing the C-terminal TEV cleavage sit and His-tag
–pFastBac 1-Gus control plasmid (control expression vector)
–Other kit reagents: 10X PCR, dNTP mix, Salt solution, sterile water, control PCR template, polyhedrin forward primer, SV40 pA reverse primer
- One Shot Mach1-T1R Chemically Competent E. coli for 20 reactions: 20 x 50 µL
- MAX Efficiency DH10Bac Competent E. coli for 20 reactions: 4 kits with 5 x 0.1 mL each
- ExpiFectamine Sf Transfection Reagent: 1 mL