Gibco™ Expi293™ GnTI- Expression System Kit

Gibco™ Expi293™ GnTI- Expression System Kit

The Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The Expi293F GnTI- cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Features

• Homogeneous N-glycosylation of expressed proteins
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
• Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Contents

• Expi293F GnTI- Cells (1x107 cells/vial)
• Expi293 Expression Medium: 1 L 
• ExpiFectamine 293 Transfection Kit for 1 L of culture
• Opti-Plex Complexation Buffer: 100 mL 
• PNGase F Glycan Cleavage Kit
• pRABBIT IgG IRES-EmGFP Positive Control Vector