Gibco™ BGJb Medium

Gibco™ BGJb Medium

BGJb medium was developed for the growth of fetal rat long bones in a chemically defined medium. BGJb is a medium modified by Sylvia Fitton-Jackson from the original BGJ medium developed by Biggers, Gwatkin, and Judah. BGJb medium creates favorable conditions for growth and calcification of fetal bones

Features

• WithL-glutamine, Phenol Red
• Without HEPES

Using BGJb medium

• BGJb contains no proteins, lipids, or growth factors. Therefore, BGJb requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BGJb uses a sodium bicarbonate buffer system (3.5 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH