Gibco™ Blasticidin S HCl, powder, 50 mg

Gibco™ Blasticidin S HCl, powder, 50 mg

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week