Gibco™ Diploid Production Serum-Free Medium (SFM), 100 L

Gibco™ Diploid Production Serum-Free Medium (SFM), 100 L

The Gibco Diploid Serum-Reduced Medium (SRM) system is designed to support vaccine manufacture under serum-reduced or serum-free conditions for cell types traditionally cultured in classical media with 10% serum. With this system, human diploid cells, such as MRC-5, Wi38, and KMB17, can be grown with just 1–2% serum. Additionally, it supports growth of chicken embryo fibroblasts under serum-free conditions. Virus production can then be conducted under serum-free conditions without the addition of albumin or other supplements. Human cells have traditionally been used to manufacture a variety of vaccines, such as the varicella zoster, polio, smallpox, hepatitis A, measles, mumps, and rubella vaccines. Traditionally, the only way to culture these cells has been to use classical media with 10% serum for growth and 1–5% human albumin (human sourced or recombinant) for virus production. These are two of the highest cost components in vaccine production. The Diploid SRM system helps reduce or eliminate both components while maintaining high virus titers

Contents

• Dry powder basal medium (store at 2–8°C)
• 100X supplement (store at -20°C)