Gibco™ Expi293™ Inducible GnTI- Expression System Kit

Gibco™ Expi293™ Inducible GnTI- Expression System Kit

The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible GnTI- cell line was established by the selective knockout of the GnTI gene and the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter, in parental Expi293F cells. This cell line is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.

Features

• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Contents

• Expi293F Inducible GnTI- Cells (1x107 cells/vial)
• Expi293 Expression Medium: 1 L
• ExpiFectamine 293 Transfection Kit for 1 L of culture
• PNGase F Glycan Cleavage Kit
• Opti-Plex Complexation Buffer: 100 mL 
• pcDNA5/TO Mammalian Expression Vector: 20 µg 
• Tetracycline Hydrochloride: • 500 mg 
• pRABBIT IgG IRES-EmGFP Positive Control Vector