Gibco™ ExpiCHO™ Expression System Kit
On demandGibco™ ExpiCHO™ Expression System Kit
The ExpiCHO™ Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S™ cells in ExpiCHO™ Expression Medium. Transient expression is powered by ExpiFectamine™ CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed. All components work together synergistically to generate 2- to 10-fold higher protein yields than the HEK293-based Expi293™ Expression System Kit and 20- to 150-fold higher yields than the FreeStyle™ MAX CHO Expression System. Expression yields of 1 to 3 g per L of transfected culture have been demonstrated for some antibody and non-antibody proteins.
Features
- Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently higher than transient 293-based systems
- Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
- A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
- Scalable expression for cultures from <1 mL to >10 L, thus generating the amount of protein needed for your application
Contents
- Frozen ExpiCHO-S cells: 2 vials
- ExpiCHO Expression Medium: 1000 mL
- ExpiFectamine CHO Transfection Kit for 1 L of culture
- Antibody Expressing Positive Control Vector: 1 vial
- OptiPRO SFM: 100 mL
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