Gibco™ Freedom™ ExpiCHO-S™ Kit

Gibco™ Freedom™ ExpiCHO-S™ Kit

The Gibco Freedom ExpiCHO-S Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for the cloning and expression of recombinant proteins in Chinese hamster ovary (CHO)-derived suspension culture. The Freedom ExpiCHO-S Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The kit also delivers high titers and offers simplified commercial licensing options

Features

• Seamless transition from transient to stable clones—all in one cell line
• Regulatory-friendly cGMP-banked cells
• Dual vector system that provides ability to control heavy- to light-chain ratios
• Ability to achieve titers of 3–5 g/L in fed batch cultures
• Short development timeline with <5 months needed to go from gene of interest to clone
• Access to technical support and consultation
• Flexible, royalty-free commercial licensing options
• Cell Line: ExpiCHO-S cells, cGMP Banked
• Promoter: CMV/EF1 Hybrid Promoter, PGK Promoter

Contents

• Gibco ExpiCHO Expression Medium, 2–8°C, protect from light
• ExpiCHO-S cells (cGMP-banked), liquid nitrogen
• One Shot TOP10 Chemically Competent E. coli, –80°C
• Freedom pCHO 3.0 Expression vectors, –5 to –20°C