Gibco™ Expi293F™ Inducible GnTI- Cells
On demand
Order number :
A39242
Gibco™ Expi293F™ Inducible GnTI- Cells
The Expi293F Inducible GnTI- cell line is derived from engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The cell line was established by the selective knockout of the GnTI gene and stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.
Features
- Suspension, high-density culture in Expi293 Expression Medium
- Homogeneous N-glycosylation of expressed proteins
- Tetracycline-inducible protein expression with extremely low basal expression levels
- Equivalent protein yields to parental Expi293F cells—up to 1 g/L
Contents
- 1 vial Expi293F Inducible GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen