Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2), PMA-free
On demand
Order number :
S0165
Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2), PMA-free
Human Melanocyte Growth Supplement-2 (HMGS-2), PMA-free, is a sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5-mL bottle of HMGS-2 contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500-mL bottle of medium.
Features
When a 500-mL bottle of medium is supplemented with HMGS-2, the final concentrations of the components in the supplemented medium are:
- Fetal bovine serum: 0.5%
- Basic fibroblast growth factor: 3 ng/mL
- Bovine pituitary extract: 0.2%
- Heparin: 3 µg/mL
- Hydrocortisone: 0.18 µg/mL
- Recombinant human insulin-like growth factor-I: 0.01 µg/mL
- Transferrin: 5 µg/mL
- Endothelin-1: 10 nM
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