Gibco™ Keratinocyte-SFM (1X), with L-glutamine, 10 x 500 mL

Gibco™ Keratinocyte-SFM (1X), with L-glutamine, 10 x 500 mL

Keratinocyte-SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. Keratinocyte-SFM is a complete serum-free medium when supplemented with human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) at the time of use. Both supplements, rEGF and BPE, are supplied in separate packaging with the basal medium. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal substitutes, in vitro toxicology, and gene therapy. This medium has been successfully used to cultivate cervical epithelial cells and is recommended for the culture of Human Corneal Epithelial Cells (HCECs). It may also be suitable as a medium for cultivating other epithelial cells, such as human bronchial epithelial cells, which have been shown to grow under serum-free conditions. Keratinocyte-SFM without calcium is available to allow researchers to adjust the Ca++ concentration to meet the demands of their specific application