Gibco™ PNGase F Glycan Cleavage Kit

Gibco™ PNGase F Glycan Cleavage Kit

The PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins. The kit includes the PNGase F enzyme, which cleaves N-glycan chains at the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.

Features

• ≥95% purity
• Recombinant PNGase F enzyme with no endoglycosidase F1, F2, or F3
• Enzyme leaves N-glycan core oligosaccharides intact and suitable for downstream analysis
• Suitable for use under native or denaturing conditions

Contents

• PNGase F enzyme, recombinant (500,000 units/mL): 33,000 units
• PNGase F 10X Buffer: 1 mL