Invitrogen™ T4 DNA Ligase Buffer

Invitrogen™ T4 DNA Ligase Buffer

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. A T4 DNA Ligase Technical Bulletin is available.

Contents

• T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6)

• 50 mM MgCl2

• 5 mM ATP

• 5 mM DTT

• 25% (w/v) polyethylene glycol-8000]