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Gibco™ DMEM/F-12, GlutaMAX™ Supplement, 500 mL

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1 bottle x 500 mL
Order number : 31331028
(Ex-work price)
$ On demand

Gibco™ DMEM/F-12, GlutaMAX™ Supplement, 500 mL

 

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications.

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12.

 

Features

  • With GlutaMAX™ & Phenol Red.
  • Without HEPES.
  • This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
  • DMEM/F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

 

Using DMEM/F-12

  • DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS).
  • DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
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