Gibco™ Medium 199, HEPES, 10 x 500 mL

Gibco™ Medium 199, HEPES, 500 mL

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications.

Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Features

• With L-glutamine, HEPES & Phenol Red.
• Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins.

Using Medium 199

• Medium 199 contains no proteins, lipids, or growth factors.
• Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.