Image Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit
On demand
Order number :
K252002
Image Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit
The pCR™8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR™8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.
Features
- Fast growing E. coli that shortens cloning time
- Maximum convenience: includes plasmid miniprep kit
- att sites for rapid recombination into any Gateway® destination vector
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
- Spectinomycin resistance gene for robust selection in E. coli
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
Contents
- pCR™8/GW/TOPO™ Vector
- dNTPs
- Salt Solution
- Sterile Water
- Gateway Forward and Reverse Sequencing Primers
- Control Template and PCR Primers
- One Shot™ TOP10 Chemically Competent E. coli
- S.O.C. Medium, and a pUC19 control plasmid.
- Resuspension Buffer
- RNase A
- Lysis Buffer
- Precipitation Buffer
- Wash Buffer
- TE Buffer
- Spin Columns
- Wash and Recovery Tubes
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