Image Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit

Image Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit

The pCR™8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR™8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

Features

• Fast growing E. coli that shortens cloning time
• Maximum convenience: includes plasmid miniprep kit
• att sites for rapid recombination into any Gateway® destination vector
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts

Contents

• pCR™8/GW/TOPO™ Vector
• dNTPs
• Salt Solution
• Sterile Water
• Gateway Forward and Reverse Sequencing Primers
• Control Template and PCR Primers
• One Shot™ TOP10 Chemically Competent E. coli
• S.O.C. Medium, and a pUC19 control plasmid. 
• Resuspension Buffer
• RNase A
• Lysis Buffer
• Precipitation Buffer
• Wash Buffer
• TE Buffer
• Spin Columns
• Wash and Recovery Tubes