Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

The pCR™8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR™8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector.

With our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

Features

• att sites for rapid recombination into a variety of Gateway® destination vectors
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoRI sites flanking the PCR product insertion site for easy excision of inserts

Contents

• TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector
• dNTPs
• Salt Solution
• Sterile Water
• Gateway Forward and Reverse Sequencing Primers
• Control Template and PCR Primers
• One Shot™ TOP10 Chemically Competent E. coli 
• S.O.C. Medium
• pUC19 control plasmid