Invitrogen 100 µg CD133 (Prominin-1) Monoclonal Antibody (13A4), Super Bright 436, eBioscience™

Invitrogen 100 µg CD133 (Prominin-1) Monoclonal Antibody (13A4), Super Bright 436, eBioscience™

The 13A4 monoclonal antibody recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural and endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown. The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen but has been reported to work in canine/dog.

• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
• Recommended Isotype Control: Rat IgG1 kappa Isotype Control (eBRG1), Super Bright 436, eBioscience™.