Invitrogen 100 µg CD45R (B220) Monoclonal Antibody (RA3-6B2), Super Bright 436, eBioscience™

Invitrogen 100 µg CD45R (B220) Monoclonal Antibody (RA3-6B2), Super Bright 436, eBioscience™

The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
• Recommended Isotype Control: Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 436, eBioscience™