Invitrogen 25 µg CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience™
On demand
Order number :
47-0441-80
Invitrogen 25 µg CD44 Monoclonal Antibody (IM7), APC-eFluor 780, eBioscience™
The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.
- APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
- Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Filtration: 0.2 µm post-manufacturing filtered.
- Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor 780, eBioscience™
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