Invitrogen 25 µg Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™
On demand
Order number :
15-4321-80
Invitrogen 25 µg Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™
The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin, This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA.
- Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
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