Invitrogen 25 µg Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610, eBioscience™

Invitrogen 25 µg Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610, eBioscience™

The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin, This eBR2a antibody has been reported for use in flow cytometric analysis, PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
• Filtration: 0.2 µm post-manufacturing filtered.