Invitrogen™ A-23187 Free Acid (Calcimycin)

Invitrogen™ A-23187 Free Acid (Calcimycin)

The Ca2+ ionophore A-23187 is commonly used for in situ calibrations of fluorescent Ca2+ indicators, to equilibrate intracellular and extracellular Ca2+ concentrations and to permit Mn2+ to enter the cell to quench intracellular dye fluorescence. Although the intrinsic fluorescence of A-23197 is too high for use with UV-excitable calcium indicators like fura-2, indo-1 and quin-2 it is suitable for use with the visible light-excitable indicators, including fluo-4, fluo-3, Calcium Green and rhod-2. A-23187 can also be used to equilibrate extracelular and intrcellular Mg2+ concentrations, making it useful for calibrating Mg2+ indicators.

For use with equipment: Fluorescence Microscope, Flow Cytometer, Fluorometer, Fluorescent Imager