Invitrogen™ Fluo-4 NW Calcium Assay Kit, 10 Microplates

Invitrogen™ Fluo-4 NW Calcium Assay Kit, 10 Microplates

Fluo-4 NW (No-Wash) Calcium Assay Kits offer a proprietary calcium assay formulation that requires neither a wash step nor a quencher dye. The fluo-4 NW assay achieves larger increases in fluorescence intensity than standard fluo-3 and fluo-4 assays with a wash step. Eliminating the wash step results in lower variability and higher Z´ values than the standard fluo-4 assay, while providing an easier and faster assay as well.

The fluo-4NW indicator is nonfluorescent and stable in pH 7–7.5 buffer for several hours, so spontaneous conversion to the Ca2+-sensitive form is not a significant source of background fluorescence. Contributions to baseline fluorescence by the growth medium (e.g., esterase activity, proteins interacting with receptors of interest, or phenol red) are eliminated by removing the medium prior to adding the indicator dye to the wells.

For use with equipment: Microplate Reader