Invitrogen™ Adiponectin Rat ELISA Kit

 Invitrogen™ Adiponectin Rat ELISA Kit

The Rat Adiponectin ELISA quantitates Rat Adiponectin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rat Adiponectin.

Principle of the method

The Rat Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 4.9%
• Intraassay CV: 4.8%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Rat Adiponectin Standard, lyophilized, 1 vial
• ELISA Buffer (10X), 2 × 30 mL
• Antibody Coated Wells, 96-well plate, 1 plate
• Rat Adiponectin Detection Antibody, 1000X 20 μL
• Anti-Mouse IgG HRP (100X), 150 μL
• Wash Buffer (10X), 2 × 30 mL
• TMB Substrate Solution, 12 mL
• Stop Solution, 12 mL
• Adhesive Plate Covers, 2