Invitrogen™ Adiponectin Mouse ELISA Kit

 Invitrogen™ Adiponectin Mouse ELISA Kit

The Mouse Adiponectinn ELISA quantitates Ms Adiponectin in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Adiponectin.

Principle of the method

The Mouse Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 5.2%
• Intraassay CV: 2.6%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Ms Adiponectin Standard, lyophilized, 1 vial
• Antibody Coated Wells, 96-well plate, 1 plate
• Ms Adiponectin Detection Antibody 200X, 60 μL
• Anti-Rabbit IgG HRP 100X, 150 μL
• ELISA Buffer (10X), 2 × 30 mL
• Wash Buffer (10X), 2 × 30 mL
• TMB Substrate Solution, 12 mL
• Stop Solution, 12 mL
• Adhesive Plate Covers, 2