Invitrogen AFP Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen AFP Human ELISA Kit, 10 x 96 Tests
The Human Alpha FetoProtein (Hu AFP) ELISA quantitates Hu AFP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu AFP.
Principle of the method
The Human AFP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human Alpha-fetoprotein Antibody Coated wells, 96-well plate, 1 plate
- Human Alpha-fetoprotein Biotin Conjugate, 2 vials
- Human Alpha-fetoprotein Standard, recombinant human alpha-fetoprotein, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (700X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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