Invitrogen Aggrecan (PG) Human ELISA Kit, 96 Tests

Invitrogen Aggrecan (PG) Human ELISA Kit, 96 Tests

The Human Aggrecan (PG) ELISA quantitates Hu Aggrecan (PG) in humansynovial fluid, serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Aggrecan (PG).

Principle of the method

The Human Aggrecan (PG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second enzyme-conjugated (detector) antibody, binding to the target on a different epitope from the capture antibody. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 5.6%
• Intraassay CV: 2.1%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard
• Sample diluent
• Incubation Buffer
• HRP conjugated Detection Antibody
• Conjugate Diluent
• Wash Buffer
• Chromogen
• Stop Solution
• Controls