Invitrogen AKT Multispecies In-Cell ELISA Kit, Colorimetric, 96 Tests

Invitrogen AKT Multispecies In-Cell ELISA Kit, Colorimetric, 96 Tests

The AKT Multispecies In-Cell ELISA Colorimetric Detection Kit is a simple method for quantifying intracellular proteins in whole cells.

Principle of the method
To perform the assay, cells are first plated, treated and fixed. Expression of the protein(s) of interest is monitored in wells of a microplate using target-specific primary antibodies (see the Important Product Information section for antibodies included in each kit) and a horseradish peroxidase (HRP)-conjugated detection reagent. The kit is supplied with a whole-cell stain to control for differences in cell plating, which is important when measuring relative levels of a protein with different treatments or assessing its post-translational modification (PTM) form. After staining, the results are analyzed by normalizing the absorbance (HRP activity) values to cell number, which adjusts for the cell plating differences among the wells.

Features

• Homogenous (no wash): No
• Storage: 2-8°C

Contents

• Blocking Buffer, 50mL
• 20X Tris Buffered Saline, 50mL
• Surfact-Amps 20 (10% Tween™ 20 Detergent), 10mL
• Surfact-Amps X-100 (10% Triton™ X-100 Detergent), 10mL
• HRP Conjugate, 0.14mL
• TMB Substrate, 58mL
• TMB Stop Solution, 55mL
• Janus Green Whole-Cell Stain, 50mL
• Elution Buffer, 2 × 30mL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho AKT (S473) Antibody
• Anti-AKT Antibody