Invitrogen AKT (Total/Phospho) Multispecies InstantOne™ ELISA Kit, 96 Tests
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Order number :
85-86046-11
Invitrogen AKT (Total/Phospho) Multispecies InstantOne™ ELISA Kit, 96 Tests
The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Ser473) in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Features
- Homogenous (no wash): No
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- User Manual
- AKT 1/2/3 (Total) Capture Antibody Reagent (Part No. IOAKTBCAR1M) – 1 x 1.5 mL
- AKT 1/2/3 (Total) Detection Antibody Reagent (Part No. IOAKTBDAR1M) – 1 x 1.5 mL
- phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent (Part No. IOPAKTACAR1M) – 1 x 1.5 mL
- phospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent (Part No. IOPAKTADAR1M) – 1 x 1.5 mL
- InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed)
- Cell Lysis Buffer (5X) (Part No. IOCLB1) - 12 mL
- Enhancer Solution (Part No. IOES1) - 1 mL
- Wash Buffer (10X) (Part No. IOWB1) - 15 mL
- Detection Reagent (TMB Substrate) (Part No. IODR1) - 12 mL
- Stop Solution (Part No. IOSS1) - 12 mL
- Positive Control Cell Lysate (lyophilized) (Part No. IOAKTAPC1) – 0.25 mL
- Plate seal - 1 ea
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