Invitrogen™ Alexa Fluor™ 488 Protein Labeling Kit
On demandInvitrogen™ Alexa Fluor™ 488 Protein Labeling Kit
The Alexa Fluor 488 Protein Labeling Kit provides the materials needed to conveniently label an antibody or protein with amine-reactive green fluorescent Alexa Fluor 488 dye (ex/em 495/519 nm). This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 488. Comparably small amounts of other proteins (>20 kDa) can also be labeled. Each kit contains everything needed for three labeling reactions and subsequent purification for free dye removal. Our protein labeling kits include an easy-to-use pre-packed spin column for rapid dye removal and high antibody recovery typically greater than 85%. Stabilizing proteins must be removed from the sample before labeling.
Features
- Convenient—labeled proteins typically ready to use in 90 min (∼15 min hands-on time)
- High conjugate recovery—pre-packed spin columns generally result in >85 % recovery of antibody conjugate
- Simple protocol—react, separate, use; detailed instructions included for optional degree of labeling (DOL) determination
- Rapid purification—quickly remove unbound dye with included pre-packed spin columns in under 15 minutes
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